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Location: Portugal
Work: Microbial Biofuels Bioprocesses Monitoring and control - Flow Cytometry
Biographical: CURRICULUM VITAE PERSONAL DETAILS NAME Maria Teresa Saraiva Lopes da Silva NATIONALITY Portuguese Citizen MARITAL STATUS Married DATE OF BIRTH 28th October 1962 ADDRESS Home Rua Tomás de Figueiredo, 18,8ºEsq, 1500-599 Lisboa Portugal Work Instituto Nacional de Engenharia, Tecnologia e Inovação Departamento de Biotecnologia /Edifício F Estrada do Paço do Lumiar, 22 1649-038 Lisboa Portugal TELEPHONE + 351 21 0924600/ +351966292175 E-MAIL teresa.lopeLopes da Silva@ineti.pt EDUCATION Degree in Chemical Engineering, IST, Portugal, 1987 MSc in Biotechnology (Biochemical Engineering), IST, 2001. Ph.D. in Chemistry, University of Évora, Portugal, 2005 (Sponsored by Fundação para a Ciência e a Tecnologia) Post-doc, School of Biological Sciences, University of Birmingham, United Kingdom, 2006 (Sponsored by Fundação Calouste Gulbenkian) PRSENT RESEARCH INTERESTS Study of the interaction of the micro-organisms with the process environment using non-invasive techniques such as Flow Cytometry. Microbiology and Biochemical Engineering are used to develop knowledge on stress affected cells in the environment and process conditions during growth. Valorization of agro-industrial wastes. Single microbial cell oils. Fine lipids namely long-chain polyunsaturated fatty acids. Biofuels from microalgae and yeasts. RESEARCH "Tomato pomace biotechnological utilization" Sumate Concentrados e Sumos S.A. Company Grant (Programa 3, PEDIP). January 1992 to June 1993. “Intercomparision Study Dietary Fiber Reference Materials” Community Bureau of Reference, Contract Nº MATI-CT92-0034, 1993–1994. “Enhanced, Intelligent processing of Food and Related Wastes using Thermophilic Populations”. Commission of the European Communities, The Fifth Framework Programme, Project QLK3-1999-00004, 2000-2004. “Enhancement of bubble and drop mass transfer processes using additives”. Fundação para a Ciência e a Tecnologia, Sapiens Project N 47689, POCTI/EQU/47689/2002. VALUVA “Up-grading of wine-making industry by- products” Company grant, sponsored by UNICER, 2005-2006. “Sustainable Microbial and Biocatalytic Production of Advanced Functional Materials”, European Commission, 6th Framework Programme, FP6-2004-NMP-NI-4, 2006-2010 AMBSOTEN PORTUGAL –Industrial Project, 2007-2008 NIDOS-NEW IDO (indoleamine 2,3-dioxygenase) INIBITORS FROM MARINE SPONGES OF ERYLUS GENUS. Sponsored by Fundação para a Ciência e a Tecnologia PTDC/QUI-QUI/098053/2008. Start: 2010. AlgaBioFuels. MICROALGAE AS A SUSTAINABLE RAW MATERIAL FOR BIOFUELS PRODUCTION (BIODIESEL, BIOETHANOL, BIO-H2 AND BIOGAS). Sponsored by Fundação para a Ciência e a Tecnologia, PTDC/AAC-AMB/100354/2008. Start: 2010. PEER REFEREE JOURNALS Lopes da Silva T., Sousa, E., Pereira, P., Ferrão, A., Roseiro, J.C. 1998. Cellular fatty acid profiles for the differentiation of Penicillium species. Fems Microbiol. Lett. 164, 303-310. Serrano, I, Lopes da Silva T.,. Roseiro, J.C. 2001. Ethanol induced dimorphism and lipid composition changes in Mucor fragilis. Lett. Appl. Microbiol. 33, 1-5. Lopes da Silva, T, Pinheiro H.M., Roseiro, JC. 2003. Stress induced morphological and physiological changes in gamma-linolenic acid production Mucor fragilis in batch and continuous cultures. Enzyme Microb. Technol. 32, 880-888. Reis, A., Lopes da Silva T., Kent, C., Kosseva, M.R., Roseiro, J. Hewitt C.J. 2005. Monitoring population dynamics of the thermophilic Bacillus licheniformis CCMI 1034 in batch and continuous cultures using multi-parameter flow cytometry. J. Biotechnol. 115, 199-210. Lopes da Silva T., Reis, A., Kent, C.A., Kosseva, M.R., Roseiro, J.C., Hewitt, C.J. 2005. Stress-induced physiological responses to glucose and lactose pulses in Bacillus licheniformis continuous culture fermentation processes as measured by multi-parameter flow cytometry. Biochem. Eng. J. 24, 31-41. Lopes da Silva T., Reis, A., Kent, C.A., Roseiro, J.C., Hewitt, C.J. 2005. The use of flow cytometry to study the impact of limiting nutrient, agitation and dilution rate on Bacillus licheniformis CCMI 1034 aerobic continuous cultures. Biotechnol. Bioeng., 92, 568-578. Oténio, M, Lopes da Silva T., Marques, ML, Roseiro, JC, Bidoia E. 2005. Benzene, toluene and xylene biodegradation by Pseudomonas Putida CCMI 852. Brazilian J. Microbiol., 36, 258-261 Lopes da Silva T., Santo, F.E., Pereira, P., Roseiro, J.C. 2006. Phenotypic characterization of food waste degrading Bacillus strains isolated from aerobic bioreactors. J. Basic Microbiol., 46, 1, 34-46. Lopes da Silva T., Mendes, A., Mendes, R., Alves S., Vasconcelos, J. and Reis, A. 2006. Effect of n-dodecane on Crypthecodinium cohnii fermentations and DHA production. J. Ind. Biotech. Microbiol., 33, 408-416. Lopes da Silva T., Calado, V., Lopes da Silva, N., Mendes, R.L., Alves, S.S., Vasconcelos, J.M.T. and Reis A. 2006 Effects of hydrocarbon additions on gas-liquid mass transfer coefficients in biphasic bioreactors. Biotechnol. Bioproc Eng., 11, 245-250. Mendes, A., Lopes da Silva T. and Reis, A. 2007. DHA Concentration and Purification from the Marine Heterotrophic Microalga Crypthecodinium cohnii CCMP 316 by Winterization and Urea Complexation. Food Technol. Biotechnol. 45 (1) 38-44. Mendes, A, Guerra, P, Madeira, P., Ruano, F., Lopes da Silva T. and Reis, A. 2007. Study of the DHA production by the heterotrophic microalga Crypthecodinium Cohnii CCMP 316 using carob pulp as a promising carbon source. World J . Microbiol. Biotechnol. 23, 1209-1215. Calado, R., Vitorino A., Reis, A., Lopes da Silva T. and Dinis M.T. 2008. Effect of different diets on larval production, quality and fatty acid profile of the marine ornamental shrimp Lysmata amboinensis . Aquaculture Nutrition, In Press. Lopes da Silva T., Bernardo, E., Nobre, B., Mendes, R.L. and Reis, A. 2008. Extraction of Victoria and Red Globe grape seed oils using supercritical carbon dioxide with and without ethanol. J. Food Lipids, in Press. Lopes da Silva, T. and Reis A. 2008. The use of multi-parameter flow cytometry to study the impact of n-dodecane additions to marine dinoflagellate microalga Crypthecodinium cohnii batch fermentations and DHA production. J. Ind. Microbiol. Biotechnol., In Press. Mendes, A., Reis, A., Vasconcelos R., Guerra, P. and Lopes da Silva, T. 2008. Crypthecodinium cohnii with emphasis on DHA production: a review. J. Appl. Phycol., In Press. Lopes da Silva, T., Reis, A., Roseiro, J. and Hewitt C.J. 2008. Physiological effects of the addition of n-dodecane as an oxygen vector during steady-state Bacillus licheniformis thermophillic fermentations perturbed by a starvation period or a glucose pulse. Biochemical Engineering Journal, In Press. Lopes da Silva T, Reis, A., Medeiros, R., Oliveira,C. and Gouveia, L. 2009. Monitoring oil production towards biofuel from autotrophic microalgae semi-continuous cultivations by flow cytometry. Appl. Biochem. Biotechnol.,159, 568-578. Lopes da Silva L, Santos C and Reis A. 2009 Multi-parameter flow cytometry as a tool to monitor heterotrophic microalgal batch fermentations for oil production towards biodiesel. Biotechnol. Bioproc. Eng., 14, 330-337. Lopes da Silva T, Pieckova L, Mileu J, Roseiro JC. 2009. A comparative study using the dual staining flow cytometric protocol applied to Lactobacillus rhamnosus and Bacillus licheniformis batch cultures. Enzyme Microbial Technol., 45, 134-138. doi:10.1016/j.enzmictec.2009.03.001 . 2Gouveia L, Marques A, da Lopes da Silva L and Reis A. 2009. Neochloris oleabundans UTEX # 1185: a suitable renewable lipid source for biofuel production. J. Ind. Microbiol. Biotechnol, 36, 821-826. PLATFORM PRESENTATIONS Lopes da Silva, T. 2005. Citometria de Fluxo aplicada a Bioprocessos: Uma Nova Era na Biotecnologia. 6 Apil, Campus de Gambelas, Universidade do Algarve (INVITED). Lopes da Silva, T.L. 2006 Aplicação da Citometria de Fluxo no estudo da fisioliga de biofilmes. 10 March, s de Engenharia Biotecnológica, Campus de Gambelas, Universidade do Algarve (INVITED). Lopes da Silva T. 2009. Multi-parameter flow cytometry as a tool to monitor hterotrophic microalgal fermentations for oil production towards biodiesel. 8 de Maio. Sessão Plenária “Processos Biotecnológicos”, XI Congresso de Citometria, Universidade da Beira Interior, Covilhã. Reis, A., Lopes da Silva, T.L, Mendes, R., Calado, V., Lopes da Silva, N., Mendes, A, Alves, S., Vasconcelos, J. 2005. Preliminary Study of Pufa Production by Microalgae and Cyanobacteria Using Two-phase System. 2nd Mercosur Congress on Chemical Engineering, 14-18th August, Rio de Janeiro, Brazil. Lopes da Silva, T., Santo, F., Pereira, P., Roseiro, J. 2005. Phenotypic Characterization Of Thermophilic Bacillus Strains Isolated From Aerobic Waste Treatment Bioreactors. 2nd Mercosul Congress on Chemical Engineering, 14-18th August, Rio de Janeiro, Brazil. Lopes da Silva, T.., Reis, A., Hewitt, C.J. , Kent, C.A., Roseiro. 2005. The use of multi-parameter flow cytometry to study the impact of limiting substrate, agitation and dilution rate on bacillus licheniformis CCMI 1034 aerobic continuous culture fermentations. 2nd Mercosur Congress on Chemical Engineering, 14-18 August, Rio de Janeiro, Brazil. POSTERS PRESENTATIONS Lopes da Silva, T., Marques, M.L. e Quinta , M.L.. 1990. ”Introdução ao Estudo de Compostos Catiónicos e sua Biodegradação“. 2ª Conferência sobre a Qualidade do Ambiente, 4-6 Abril, Lisbon, Portugal. Lopes da Silva, T., Sousa, E., Pereira, P., Ferrão, A. e Roseiro, J.C. 1997. Statistical Comparison of Fatty Acid Composition of Penicillium Species using Multivariate Analysis”. XVI Annual Meeting of European Culture Collections Organization, 26-28 Junho, Estoril, Portugal. Machado, A., Antunes, C., Lopes da Silva, T., Roseiro, J.C. 2001. “Optimização e desenvolvimento de um meio de crescimento definido para Bacillus stearothemophilus CCMI 237”. Micro2001, 5 a 8 de Dezembro, Póvoa do Varzim, Portugal. Otenio, M.H., Lopes da Silva, T. E.D. Bidóia e Roseiro, J.C. 2001.”BTX, biodegradação, cometabolismo e inibição por Pseudomonas putida CCMI 852. Micro’2001, 5 a 8 de Dezembro, Póvoa do Varzim, Portugal. Lopes da Silva, T., Reis, A., Hewitt, C.J. , Kent, C.A., Roseiro, J.C. 2003. Bacillus licheniformis CCMI 1034 steady-state continuous cultures sub-populations analysed by multiparameter flow cytometry. Micro2003, 29 Novembro a 2 Dezembro, Tomar, Portugal. Lopes da Silva, T., Reis, A., Hewitt, C.J., Kent, C.A. Roseiro, J.C. 2003. Physiological responses to carbon pulses and sub-populations characterization of Bacillus licheniformis CCMI 1034 cultures analysed by multiparameter flow cytometry. Micro2003, 29 Novembro a 2 Dezembro,Tomar, Portugal. Reis, A. Lopes da Silva, T., Kent, C.A., Kosseva M.R., Roseiro, J.C., Hewitt, C.J. 2004.Stress-induced physiological responses to carbon substrate pulses in Bacillus licheniformis cultures as measured by multi-parameter flow cytometry. European Symposium on Biochemical Engineering Science, 8-11 September, Stuttgart, Germany. Lopes da Silva, T.L., Reis, A., Kent, C.A., Kosseva M.R., Roseiro, J.C., Hewitt, C.J. 2004. Monitoring population dynamics of the thermophilic Bacillus licheniformis CCMI 1034 in batch and continuous cultures using multi-parameter flow cytometry. European Symposium on Biochemical Engineering Science, 8-11 September, Stuttgart, Germany.
Favourite Publications: Biotechnology and Bioengineering Journal of Biotechnolgy
Work: Microbial Biofuels Bioprocesses Monitoring and control - Flow Cytometry
Biographical: CURRICULUM VITAE PERSONAL DETAILS NAME Maria Teresa Saraiva Lopes da Silva NATIONALITY Portuguese Citizen MARITAL STATUS Married DATE OF BIRTH 28th October 1962 ADDRESS Home Rua Tomás de Figueiredo, 18,8ºEsq, 1500-599 Lisboa Portugal Work Instituto Nacional de Engenharia, Tecnologia e Inovação Departamento de Biotecnologia /Edifício F Estrada do Paço do Lumiar, 22 1649-038 Lisboa Portugal TELEPHONE + 351 21 0924600/ +351966292175 E-MAIL teresa.lopeLopes da Silva@ineti.pt EDUCATION Degree in Chemical Engineering, IST, Portugal, 1987 MSc in Biotechnology (Biochemical Engineering), IST, 2001. Ph.D. in Chemistry, University of Évora, Portugal, 2005 (Sponsored by Fundação para a Ciência e a Tecnologia) Post-doc, School of Biological Sciences, University of Birmingham, United Kingdom, 2006 (Sponsored by Fundação Calouste Gulbenkian) PRSENT RESEARCH INTERESTS Study of the interaction of the micro-organisms with the process environment using non-invasive techniques such as Flow Cytometry. Microbiology and Biochemical Engineering are used to develop knowledge on stress affected cells in the environment and process conditions during growth. Valorization of agro-industrial wastes. Single microbial cell oils. Fine lipids namely long-chain polyunsaturated fatty acids. Biofuels from microalgae and yeasts. RESEARCH "Tomato pomace biotechnological utilization" Sumate Concentrados e Sumos S.A. Company Grant (Programa 3, PEDIP). January 1992 to June 1993. “Intercomparision Study Dietary Fiber Reference Materials” Community Bureau of Reference, Contract Nº MATI-CT92-0034, 1993–1994. “Enhanced, Intelligent processing of Food and Related Wastes using Thermophilic Populations”. Commission of the European Communities, The Fifth Framework Programme, Project QLK3-1999-00004, 2000-2004. “Enhancement of bubble and drop mass transfer processes using additives”. Fundação para a Ciência e a Tecnologia, Sapiens Project N 47689, POCTI/EQU/47689/2002. VALUVA “Up-grading of wine-making industry by- products” Company grant, sponsored by UNICER, 2005-2006. “Sustainable Microbial and Biocatalytic Production of Advanced Functional Materials”, European Commission, 6th Framework Programme, FP6-2004-NMP-NI-4, 2006-2010 AMBSOTEN PORTUGAL –Industrial Project, 2007-2008 NIDOS-NEW IDO (indoleamine 2,3-dioxygenase) INIBITORS FROM MARINE SPONGES OF ERYLUS GENUS. Sponsored by Fundação para a Ciência e a Tecnologia PTDC/QUI-QUI/098053/2008. Start: 2010. AlgaBioFuels. MICROALGAE AS A SUSTAINABLE RAW MATERIAL FOR BIOFUELS PRODUCTION (BIODIESEL, BIOETHANOL, BIO-H2 AND BIOGAS). Sponsored by Fundação para a Ciência e a Tecnologia, PTDC/AAC-AMB/100354/2008. Start: 2010. PEER REFEREE JOURNALS Lopes da Silva T., Sousa, E., Pereira, P., Ferrão, A., Roseiro, J.C. 1998. Cellular fatty acid profiles for the differentiation of Penicillium species. Fems Microbiol. Lett. 164, 303-310. Serrano, I, Lopes da Silva T.,. Roseiro, J.C. 2001. Ethanol induced dimorphism and lipid composition changes in Mucor fragilis. Lett. Appl. Microbiol. 33, 1-5. Lopes da Silva, T, Pinheiro H.M., Roseiro, JC. 2003. Stress induced morphological and physiological changes in gamma-linolenic acid production Mucor fragilis in batch and continuous cultures. Enzyme Microb. Technol. 32, 880-888. Reis, A., Lopes da Silva T., Kent, C., Kosseva, M.R., Roseiro, J. Hewitt C.J. 2005. Monitoring population dynamics of the thermophilic Bacillus licheniformis CCMI 1034 in batch and continuous cultures using multi-parameter flow cytometry. J. Biotechnol. 115, 199-210. Lopes da Silva T., Reis, A., Kent, C.A., Kosseva, M.R., Roseiro, J.C., Hewitt, C.J. 2005. Stress-induced physiological responses to glucose and lactose pulses in Bacillus licheniformis continuous culture fermentation processes as measured by multi-parameter flow cytometry. Biochem. Eng. J. 24, 31-41. Lopes da Silva T., Reis, A., Kent, C.A., Roseiro, J.C., Hewitt, C.J. 2005. The use of flow cytometry to study the impact of limiting nutrient, agitation and dilution rate on Bacillus licheniformis CCMI 1034 aerobic continuous cultures. Biotechnol. Bioeng., 92, 568-578. Oténio, M, Lopes da Silva T., Marques, ML, Roseiro, JC, Bidoia E. 2005. Benzene, toluene and xylene biodegradation by Pseudomonas Putida CCMI 852. Brazilian J. Microbiol., 36, 258-261 Lopes da Silva T., Santo, F.E., Pereira, P., Roseiro, J.C. 2006. Phenotypic characterization of food waste degrading Bacillus strains isolated from aerobic bioreactors. J. Basic Microbiol., 46, 1, 34-46. Lopes da Silva T., Mendes, A., Mendes, R., Alves S., Vasconcelos, J. and Reis, A. 2006. Effect of n-dodecane on Crypthecodinium cohnii fermentations and DHA production. J. Ind. Biotech. Microbiol., 33, 408-416. Lopes da Silva T., Calado, V., Lopes da Silva, N., Mendes, R.L., Alves, S.S., Vasconcelos, J.M.T. and Reis A. 2006 Effects of hydrocarbon additions on gas-liquid mass transfer coefficients in biphasic bioreactors. Biotechnol. Bioproc Eng., 11, 245-250. Mendes, A., Lopes da Silva T. and Reis, A. 2007. DHA Concentration and Purification from the Marine Heterotrophic Microalga Crypthecodinium cohnii CCMP 316 by Winterization and Urea Complexation. Food Technol. Biotechnol. 45 (1) 38-44. Mendes, A, Guerra, P, Madeira, P., Ruano, F., Lopes da Silva T. and Reis, A. 2007. Study of the DHA production by the heterotrophic microalga Crypthecodinium Cohnii CCMP 316 using carob pulp as a promising carbon source. World J . Microbiol. Biotechnol. 23, 1209-1215. Calado, R., Vitorino A., Reis, A., Lopes da Silva T. and Dinis M.T. 2008. Effect of different diets on larval production, quality and fatty acid profile of the marine ornamental shrimp Lysmata amboinensis . Aquaculture Nutrition, In Press. Lopes da Silva T., Bernardo, E., Nobre, B., Mendes, R.L. and Reis, A. 2008. Extraction of Victoria and Red Globe grape seed oils using supercritical carbon dioxide with and without ethanol. J. Food Lipids, in Press. Lopes da Silva, T. and Reis A. 2008. The use of multi-parameter flow cytometry to study the impact of n-dodecane additions to marine dinoflagellate microalga Crypthecodinium cohnii batch fermentations and DHA production. J. Ind. Microbiol. Biotechnol., In Press. Mendes, A., Reis, A., Vasconcelos R., Guerra, P. and Lopes da Silva, T. 2008. Crypthecodinium cohnii with emphasis on DHA production: a review. J. Appl. Phycol., In Press. Lopes da Silva, T., Reis, A., Roseiro, J. and Hewitt C.J. 2008. Physiological effects of the addition of n-dodecane as an oxygen vector during steady-state Bacillus licheniformis thermophillic fermentations perturbed by a starvation period or a glucose pulse. Biochemical Engineering Journal, In Press. Lopes da Silva T, Reis, A., Medeiros, R., Oliveira,C. and Gouveia, L. 2009. Monitoring oil production towards biofuel from autotrophic microalgae semi-continuous cultivations by flow cytometry. Appl. Biochem. Biotechnol.,159, 568-578. Lopes da Silva L, Santos C and Reis A. 2009 Multi-parameter flow cytometry as a tool to monitor heterotrophic microalgal batch fermentations for oil production towards biodiesel. Biotechnol. Bioproc. Eng., 14, 330-337. Lopes da Silva T, Pieckova L, Mileu J, Roseiro JC. 2009. A comparative study using the dual staining flow cytometric protocol applied to Lactobacillus rhamnosus and Bacillus licheniformis batch cultures. Enzyme Microbial Technol., 45, 134-138. doi:10.1016/j.enzmictec.2009.03.001 . 2Gouveia L, Marques A, da Lopes da Silva L and Reis A. 2009. Neochloris oleabundans UTEX # 1185: a suitable renewable lipid source for biofuel production. J. Ind. Microbiol. Biotechnol, 36, 821-826. PLATFORM PRESENTATIONS Lopes da Silva, T. 2005. Citometria de Fluxo aplicada a Bioprocessos: Uma Nova Era na Biotecnologia. 6 Apil, Campus de Gambelas, Universidade do Algarve (INVITED). Lopes da Silva, T.L. 2006 Aplicação da Citometria de Fluxo no estudo da fisioliga de biofilmes. 10 March, s de Engenharia Biotecnológica, Campus de Gambelas, Universidade do Algarve (INVITED). Lopes da Silva T. 2009. Multi-parameter flow cytometry as a tool to monitor hterotrophic microalgal fermentations for oil production towards biodiesel. 8 de Maio. Sessão Plenária “Processos Biotecnológicos”, XI Congresso de Citometria, Universidade da Beira Interior, Covilhã. Reis, A., Lopes da Silva, T.L, Mendes, R., Calado, V., Lopes da Silva, N., Mendes, A, Alves, S., Vasconcelos, J. 2005. Preliminary Study of Pufa Production by Microalgae and Cyanobacteria Using Two-phase System. 2nd Mercosur Congress on Chemical Engineering, 14-18th August, Rio de Janeiro, Brazil. Lopes da Silva, T., Santo, F., Pereira, P., Roseiro, J. 2005. Phenotypic Characterization Of Thermophilic Bacillus Strains Isolated From Aerobic Waste Treatment Bioreactors. 2nd Mercosul Congress on Chemical Engineering, 14-18th August, Rio de Janeiro, Brazil. Lopes da Silva, T.., Reis, A., Hewitt, C.J. , Kent, C.A., Roseiro. 2005. The use of multi-parameter flow cytometry to study the impact of limiting substrate, agitation and dilution rate on bacillus licheniformis CCMI 1034 aerobic continuous culture fermentations. 2nd Mercosur Congress on Chemical Engineering, 14-18 August, Rio de Janeiro, Brazil. POSTERS PRESENTATIONS Lopes da Silva, T., Marques, M.L. e Quinta , M.L.. 1990. ”Introdução ao Estudo de Compostos Catiónicos e sua Biodegradação“. 2ª Conferência sobre a Qualidade do Ambiente, 4-6 Abril, Lisbon, Portugal. Lopes da Silva, T., Sousa, E., Pereira, P., Ferrão, A. e Roseiro, J.C. 1997. Statistical Comparison of Fatty Acid Composition of Penicillium Species using Multivariate Analysis”. XVI Annual Meeting of European Culture Collections Organization, 26-28 Junho, Estoril, Portugal. Machado, A., Antunes, C., Lopes da Silva, T., Roseiro, J.C. 2001. “Optimização e desenvolvimento de um meio de crescimento definido para Bacillus stearothemophilus CCMI 237”. Micro2001, 5 a 8 de Dezembro, Póvoa do Varzim, Portugal. Otenio, M.H., Lopes da Silva, T. E.D. Bidóia e Roseiro, J.C. 2001.”BTX, biodegradação, cometabolismo e inibição por Pseudomonas putida CCMI 852. Micro’2001, 5 a 8 de Dezembro, Póvoa do Varzim, Portugal. Lopes da Silva, T., Reis, A., Hewitt, C.J. , Kent, C.A., Roseiro, J.C. 2003. Bacillus licheniformis CCMI 1034 steady-state continuous cultures sub-populations analysed by multiparameter flow cytometry. Micro2003, 29 Novembro a 2 Dezembro, Tomar, Portugal. Lopes da Silva, T., Reis, A., Hewitt, C.J., Kent, C.A. Roseiro, J.C. 2003. Physiological responses to carbon pulses and sub-populations characterization of Bacillus licheniformis CCMI 1034 cultures analysed by multiparameter flow cytometry. Micro2003, 29 Novembro a 2 Dezembro,Tomar, Portugal. Reis, A. Lopes da Silva, T., Kent, C.A., Kosseva M.R., Roseiro, J.C., Hewitt, C.J. 2004.Stress-induced physiological responses to carbon substrate pulses in Bacillus licheniformis cultures as measured by multi-parameter flow cytometry. European Symposium on Biochemical Engineering Science, 8-11 September, Stuttgart, Germany. Lopes da Silva, T.L., Reis, A., Kent, C.A., Kosseva M.R., Roseiro, J.C., Hewitt, C.J. 2004. Monitoring population dynamics of the thermophilic Bacillus licheniformis CCMI 1034 in batch and continuous cultures using multi-parameter flow cytometry. European Symposium on Biochemical Engineering Science, 8-11 September, Stuttgart, Germany.
Favourite Publications: Biotechnology and Bioengineering Journal of Biotechnolgy
